RESPIRATORY SYSTEM IN ANIMAL( INSECT

RESPIRATORY SYSTEM IN ANIMAL( INSECT)

Insects breathe using air tubes called trachea. Air vessel out into the trachea through a small hole every body segments called stigma or spiracles. The air from the spiracles through the trachea, leading to trakeol and trakeolus.

1. Aim     :

1.       To  p rove that the insects need oxygen  for respiration

2.       To  understand the factors that affect the amount of oxygen demand on insects during breathing

3.       To Calculate the average respiration in insects ml per minute

2. Apparatus and Materials:

1.    Stop Watch

2.    Respirometer

3.    Vaseline

4.    NaOH/KOH(crystal)

5.    Eosin

6.    Crickets/ grasshopper

7.    Pipette

8.    Balance

III.   Procedure

1.    Prepare  the required apparatus and materials

2.    Wrapping NaOH crystals by using a cotton swab and put it in the respirometer

3.Measure   weight of  insects by using a scale / balance sheet , then   enter 1 cricket and closed respirometer by giving vaseline to make free from air

4.    drop eosin  by using pipette in hole  of  respirometer tube

5.                          write down result of observation  in the table of observations

Table      

No	Body Weight (gr)	Decrease  volume (ml)  each 2 minutes periods …….				Total volume
		1	2	3	4

6.    Make a graph that shows the relationship between body weight crickets with the required amount of oxygen to breathe

IV.  Discussion  :

1.    What  the function of  eosin and NaOH/KOH  in experiment?

2.    why eosin moving towards cricket?

3.    What are the factors that influence  move  position  of eosin in the pipeline?